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| Oligonucleotide synthesis |
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| (Agilent, Synteni, Incyte) |
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| also combinatorial; "two color" system |
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| robotic delivery of reagents |
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| no mask required (so cheaper, faster) but features |
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| bigger (150-200 m) |
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| typically uses 50-mers to 75-mers |
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| 50-mer specificity similar to full-length probes in |
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| specificity (>75% homology or 15 bp contiguous |
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| identical sequence causes 50-mer cross- |
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| hybridization) |
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