 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Gygi et al studied mRNA-protein level correlations in 106
|
|
|
|
yeast proteins and found correlation = 0.935, but 0.36
|
|
|
|
when highly expressed proteins removed; others have
|
|
|
|
found somewhat better correlations.
Dynamic range = 100
|
|
|
molecules for rare species to a few million for glycolytic
|
|
|
|
enzymes (yeast). MS detects
one billion molecules in best
|
|
|
|
cases.
|
|
|
|
post-translational modifications, differential half-life,
|
|
|
|
differential translation rates,…
|
|
|
|
there can be up to 10X variation in mRNA:protein ratios;
|
|
|
|
average in yeast = 1:4000 for abundant proteins
|
|