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| ICAT (isotope-coded affinity |
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| tages) |
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| two mixtures separately treated with two slightly |
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| different ICAT reagents, differing by 8 in MW |
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| samples then: |
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| mixed |
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| digested |
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| selectively enriched for ICAT-containing peptides |
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| separated by HPLC |
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| subjected to ESI-MS/MS |
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| identified from database |
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