BIOEN 455 homepage

When?  Winter 2012, WF 2:00-3:20 PM (lectures) and M 12:30 PM-3:20 PM (labs)

Where?  The lectures will be in Physics&Astronomy A214. The labs will be in the Foege building, either in the computer classroom in the basement for the first lab, in the Folch lab (Rm. N423C) for the PDMS molding lab, or in the Foege 1st floor student lab for the device testing lab.

Assignments and Grading: One assignment at the end of each section and lab reports. Class participation can increase your grade by as much as 0.1 points. 

Handouts: must be downloaded by student.

Additional Suggested Readings: must be downloaded by student: Review on Microfluidic Interfaces (Beebe), Review on Microfluidic Design Considerations for Cellular Studies (Beebe), Review on Microfluidic Physics (Beebe), Review on Molecular Biology Chips (Quake), Review on Optofluidics (Quake), Review on Soft-Microfluidics (Quake), Review on PDMS Microfluidics (Whitesides), Review on Microfluidics Past and Future, Review on Cell Patterning (Folch), Review on Cell Biology on a Chip (Folch), Review on Single Molecule Lab on a Chip (Craighead), Review on Microfluidics System Biology (Lee), Review_on Cell Patterning (Chen).

Important note for disabled individuals: If you would like to request academic accommodations due to a disability, please contact Disabled Student Services, 448 Schmitz, (206) 543-8924 (V/TTY). If you have a letter from Disabled Student Services indicating you have a disability that requires academic accommodations, please present the letter to me so we can discuss the accommodations you might need for the class.

Note that weeks and sections do not necessarily correspond exactly

Week / Section

Lecture
(by section)

Handouts (PDF)
(by section)

Assignments 
(by week)

Lab

(approx. schedule)

0

It's a small world
Dimensions and scaling challenges involved in going from macro to micro to nano

Section 0

Teaching Appendix

 

1

How do we make small things?
Introduction to micropatterning, micromachining, and micromolding with an emphasis on biomaterials restrictions

Section 1 slides or textbook Chapter 1

Design of a photomask

2

Micropatterning of substrates and cells
Self-assembled monolayers, chemically-bound biomolecules, biocompatible/biodegradable polymers

Section 2 slides or textbook Chapter 2

NO LAB Mon Jan 16 (MLK Holiday)

3

Microfluidics
Introduction to microfluidics, properties of biological fluids in microchannels, mathematical modeling of fluid flow

Section 3 slides or textbook Chapter 3

Photolithography #1

4

Molecular biology on a chip
Chromatographic separations on a chip, DNA prisms, deterministic lateral displacement, isoelectric focusing, free-flow electrophoresis, mass spectrometry, PCR chips

Section 4 slides or textbook Chapter 4

Photolithography #2

5

Cell-based chips for biotechnology
Miniature enzymatic assays, DNA microarrays, optical detection methods amenable to miniaturization

Section 5 slides or textbook Chapter 5

PDMS replica molding #1

6

BioMEMS for cell biology
Enabling the control of cell-substrate, cell-cell, and cell-medium interactions

 Section 6 slides or textbook Chapter 6

PDMS replica molding #2

7

Tissue microengineering
Introduction to biomimetic substrates and microscaffolds for tissue engineering applications

Section 7 slides or textbook Chapter 7

NO LAB Mon Feb 20 (President’s Day)

8

Microfabricated implants and biosensors
Implantable microelectrodes, microtweezers.

Section 8 slides or textbook Chapter 8

Device testing