BIOEN 455 homepage

When?  Winter 2009, MWF 3:30-4:20 PM (lectures) and 12:30 AM-3:20 PM (labs)

Where?  The lectures will be in Physics&Astronomy A214. All the labs will be in the Foege building Rm. N 423 (Folch lab).

Assignments and Grading: One assignment at the end of each section and a lab reports. The lab report will contain quizzes that are pertinent to the lectures and the lab teachings. Class participation can increase your grade by as much as 0.1 points. 

Handouts: must be downloaded by student.

Additional Suggested Readings: must be downloaded by student: Review on Microfluidic Interfaces (Beebe), Review on Microfluidic Design Considerations for Cellular Studies (Beebe), Review on Microfluidic Physics (Beebe), Review on Molecular Biology Chips (Quake), Review on Optofluidics (Quake), Review on Soft-Microfluidics (Quake), Review on PDMS Microfluidics (Whitesides), Review on Microfluidics Past and Future, Review on Cell Patterning (Folch), Review on Cell Biology on a Chip (Folch), Review on Single Molecule Lab on a Chip (Craighead), Review on Microfluidics System Biology (Lee), Review_on Cell Patterning (Chen).

Important note for disabled individuals: If you would like to request academic accommodations due to a disability, please contact Disabled Student Services, 448 Schmitz, (206) 543-8924 (V/TTY). If you have a letter from Disabled Student Services indicating you have a disability that requires academic accommodations, please present the letter to me so we can discuss the accommodations you might need for the class.

Note that weeks and sections do not necessarily correspond exactly

Week / Section

Lecture
(by section)

Handouts (PDF)
(by section)

Assignments 
(by week)

Lab

1

It's a small world
Dimensions and scaling challenges involved in going from macro to micro to nano

Section 1

 

2

How do we make small things?
Introduction to micropatterning, micromachining, and micromolding with an emphasis on biomaterials restrictions

Section 2

Design of a photomask

3

Micropatterning of non-conventional materials
Self-assembled monolayers, chemically-bound biomolecules, biocompatible/biodegradable polymers

Section 3

NO LAB Mon Jan 19 (MLK Holiday)

4

Microelectrochemical sensing of cell behavior
Introduction to bioelectricity, interaction of cells with electric fields, microphysiometer

Section 4

Photolithography #1

5

Microengineered biosensors
Introduction to massively parallel measurements, implantable microelectrodes, microtweezers, immunosensors

Section 5

Photolithography #2

6

Microengineering fluid flows
Introduction to microfluidics, properties of biological fluids in microchannels, mathematical modeling of fluid flow

Section 6

PDMS replica molding

7

Tissue microengineering
Introduction to biomimetic substrates, microscaffolds, cellular micropatterns

 Section 7

Device Assembly

8

Microengineering in cell biology
Enabling the control of cell-substrate, cell-cell, and cell-medium interactions

Section 8

NO LAB Mon Feb 16 (President’s Day)

9

Microengineering for Biotechnology
Introduction to enzymatic assays, DNA microarrays, optical detection methods amenable to miniaturization

Section 9

Qualitative testing -- dyes

10

The frontiers of BioMEMS
Nanolithography, biomimetic nanodevices

 Section 10

 

Quantitative testing --Proteins & fluorescence