HOME Class Schedule	Bioen 599 F, Autumn 2000 Bioengineering Principles of Physiology
	Lecture Material and Notes Week 2, Lecture 5: Chemomechanical transduction in actomyosin Michael Regnier Lecture theme and outline: The myosin motor is an enzyme that uses ATP as its fuel source. How is the energy from ATP hydrolysis transduced into work, force and relative filament displacement? ATP is not required for acto-myosin cross-bridge formation, but for cross-bridge dissociation. What is the relationship between ATPase reactions and myosin isoform? Fundamental enzyme kinetics and ATPase. Biochemical measures of myosin ATPase and the influence of actin. (Stopped/quenched flow, etc.) The fundamental cycle. Measures of acto-myosin ATPase in demembranated muscle fibers (caged compounds, etc.) Towards chemo-mechanical models of the cross-bridge cycle. This will lead into the modeling/simulation labs/problem sets. Suggested reading: see Lecture 4.
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