PABIO/ MEBI/ PHG 536
Bioinformatics and Gene Sequence Analysis
Spring  2009 References

  • Suggested Textbook: Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF 2nd ed. (2001), Wiley Interscience, New York. (University Bookstore, South Campus Center, $65).
  • On-line textbooks:.

Week 1- Introduction: Biological Sequence Information/NCBI Resources

Suggested readings:

Monday

·         Chapter 1.1. Evolution: At the Core of Molecular Change and Chapter 1.2. The Molecules of Life - Molecular and Cell Biology 4th ed., Lodish et al.,ed. 1999 New York: W H Freeman & Co

Wednesday

·         Chapter 1 - Bioinformatics and the Internet - Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001)

·         The Molecular Biology Database Collection: an online compilation of relevant database resources, Galperin, M. (2009), Nucleic Acids Research, Vol. 34, Database issue D1

·         About the National Center for Biotechnology Information - http://www.ncbi.nih.gov/About/index.html

Week 2- DNA Sequence Analysis

Suggested readings:

Monday

·         Chapter 9- Structure of Genes and Chromosomes in Molecular and Cell Biology 4th ed., Lodish et al.,ed. 1999 New York: W H Freeman & Co

Wednesday

·         Chapter 8.1- Mutations: Types and Causes in Molecular and Cell Biology 4th ed., Lodish et al.,ed. 1999 New York: W H Freeman & Co

 

 Week 3- Protein Analysis

Suggested readings:

Monday

Chapter 11 - Predictive Methods using Protein Sequences - (pp 257-258, 269-274) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001)

Molecular and Cell Biology 4th ed., Lodish et al., ed. 1999 New York: W H Freeman & Co

Chapter 3. Protein Structure and Function,

3.1. Hierarchical Structure of Proteins ,

3.4. Membrane Proteins

Chapter 17. Protein Sorting: Organelle Biogenesis and Protein Secretion

17.6. Post-Translational Modifications and Quality Control in the Rough ER

17.7. Protein Glycosylation in the ER and Golgi Complex

17.8. Golgi and Post-Golgi Protein Sorting and Proteolytic Processing

Background References (Suggested reading on protein sorting motifs)

Molecular and Cell Biology 4th ed., Lodish et al., ed. 1999 New York: W H Freeman & Co

Chapter 17. Protein Sorting: Organelle Biogenesis and Protein Secretion

17 Protein Sorting: Organelle Biogenesis and Protein Secretion,

17.1. Synthesis and Targeting of Mitochondrial and and Chloroplast Proteins,

17.2. Synthesis and Targeting of Peroxisomal Proteins,

17.4. Translocation of Secretory Proteins across the ER Membrane,

17.5. Insertion of Membrane Proteins into the ER Membrane

Week 4- BLAST Similarity Searching/ Nucleotide BLAST

Suggested readings:

  • Monday
    • What is BLAST? Basic information (NCBI)
    • The Statistics of Similarity Scores (BLAST) - A Primer (NCBI) (This is for general knowledge only - You will not be held responsible for understanding the statistics)
    • Chapter 8- Sequence Alignment and Database Searching (pp 198-208) - Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001) 

Background references:

·         BLAST Tutorial (NCBI)

·         Frequently Asked Questions (FAQs) concerning BLAST (NCBI)

·         Glossary of terms used in sequence alignment and BLAST searching

·         Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990).Basic local alignment search tool.   J.Mol.Biol. 215, 403-410.

Weeks 5,6 - Protein BLAST/Protein Motifs

Suggested readings:

  • Monday
    • Chapter 8 - Sequence Alignment and Database Searching - Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001)
    • Henikoff, S., and Henikoff, J. G. (1992). Amino acid substitution matrices from protein blocks. Proc.Natl.Acad.Sci.U.S.A. 89, 10915-10919.

Background references:

·         Prosite Database of Protein Families and Domains, Hulo et al., Nucleic Acids Research, 2006, Vol 34, Database Issue, D227-230

·         Pfam: Clans, Webtools and Services,  Finn et al., Nucleic Acids Research, 2006, Vol. 34, Database issue D247-51

·         Protein family classification based on searching a database of blocks.  Henikoff, S., and Henikoff, J. G. (1994). Genomics. 19, 97-107.

·         Increased coverage of protein families with the blocks database servers. J.G. Henikoff, E.A. Greene, S. Pietrokovski & S. Henikoff, Nucl. Acids Res. 28:228-230 (2000).

·         Blocks+: A non-redundant database of protein alignment blocks derived from multiple compilations, S. Henikoff, J.G. Henikoff & S. Pietrokovski, (1999), Bioinformatics 15(6):471-479.

·         Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 3389-3402.

·         PSI-BLAST Tutorial (NCBI)

Week 7- SNPs/ EST Databases

Suggested readings:

Background references:

·         Complementary DNA sequencing: expressed sequence tags and human genome project.  Adams et al (1991) Science 1991 Jun 21;252(5013):1651-6

Suggested readings:

  • Wednesday

Weeks 8,9 - Multiple Sequence Alignment/Phylogenetics Analysis

Suggested readings:

  • Monday
    •  Chapter 9 - Creation and Analysis of Protein Multiple Sequence Alignments - Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001)
    • General Help for Clustal W

Suggested readings:

  • Wednesday
    • Chapter 14 - Phylogenetic Analysis - Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins 2nd Edition, Baxevanis AD and Ouellette BF ed. (2001)

Background references:

    • Doolittle, RF (1981) Similar amino acid sequences: chance or common ancestry?. Science 214, 149-159.
    • Felsenstein J. (1996). Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods. Methods in Enzymology,. 266. 418-427.
    • Saitou N. (1996) Reconstruction of gene trees from sequence data. Methods in Enzymology 266, 427-449
    • Multiple sequence alignment in phylogenetic analysis. Phillips A, Janies D, Wheeler W. A review of the meaning of the best alignment. Mol Phylogenet Evol. 2000 Sep;16(3):317-30.
    • Phylogenetic Trees Made Easy: A How-To Manual, Second Edition
      by Barry G. Hall "This is a "cookbook" intended as a toll to aid beginners in creating phylogenetic trees from protein or nucleic acid sequence data. Publisher: Sinauer Associates; 2nd Bk&Cdr edition (June, 2004)   ISBN: 0878933123
    • Molecular Evolution: A Phylogenetic Approach
      by Roderic D. M. Page, Edward C. Holmes Publisher: Blackwell Publishers (October 1, 1998)  ISBN: 0865428891
    • Inferring Phylogenies
      by Joseph Felsenstein  Publisher: Sinauer Associates (
      September 4, 2003)  ISBN: 0878931775  

Week 10 – Protein Structure Analysis

Suggested readings:

  • Wednesday
    • C. Branden & J. Tooze, Introduction to protein structure, Garland Publ. Inc., New York, 1999, second edition.
    • Protein Stucture and Function (Primers in Biology) by Gregory A. Petsko, Dagmar Ringe, New Science Press, 2003.

 

 

 Revised 3/21/09 (TR)