PBIO 550/NeuB 550 Biophysics of Calcium Signaling

Spring 2013, first half of quarter (five weeks)

Meets: Mondays from 1:00 - 2:50 PM in G-417 of HSB

Instructors: Bertil Hille (hille@uw.edu; 3-8639, H-310G HSB) and
Luis Fernando Santana (santana@uw.edu; 3-0986, H-321 HSB)

Course Schedule/Information [pdf file]

Lecture schedule:

Date	Instructor	Topic		Discussion Paper
April 1	Santana	Fundamentals of fluorescence and intracellular Ca²⁺ indicators	Grynkiewicz et al. 1985. A new generation of Ca²⁺ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450.
April 8	Hille	Expressible calcium indicators	Miyawaki A, et al (1999) Dynamic and quantitative Ca²⁺ measurements using improved cameleons. PNAS. 96:2135-40.
April 15	Santana	Optogenetic regulation Ca²⁺ signaling	Jia et al. Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery. Circulation. Arrhythmia and electrophysiology. 2011;4:753-760.
April 22	Hille	Cellular calcium buffers and transport mechanisms. Kinetics and rates.	Neher E and Augustine GJ. 1992. Calcium gradients and buffers in bovine chromaffin cells. J Physiol 450: 273-301.
April 29	Santana	Local Ca²⁺ signaling	Sonkusare et al. Elementary Ca²⁺ signals through endothelial TRPV4 channels regulate vascular function Science 2012; 336:597-601
May 13	--	Paper is due.		see instructions

Course Evaluation

Last update April 22, 2013 12:35 PM