PBIO 550/NeuB 550 Biophysics of Calcium Signaling

Spring 2011, first half of quarter (five weeks)

Meets: Mondays from 1:00 - 2:50 PM in G-417 of HSB

Instructors: Bertil Hille (hille@u.washington.edu; 3-8639, H-310G HSB) and
Luis Fernando Santana (santana@u.washington.edu; 3-0986, H-321 HSB)

Course Schedule/Information [pdf file]

Lecture schedule:

Date	Instructor	Topic		Discussion Paper
March 28	Santana	Fundamentals of fluorescence and intracellular Ca2+ indicators	Grynkiewicz et al. 1985. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450.
April 4	Hille	Genetically expressible calcium indicators	Canato M et al. 2010 Massive alterations of SR free calcium in skeletal muscle fibers lacking calsequestrin revealed by a genetically encoded probe. PNAS. 107:22326-22331.
April 11	Hille	Cellular calcium buffers and transport mechanisms. Kinetics and rates.	Neher E and Augustine GJ. 1992. Calcium gradients and buffers in bovine chromaffin cells. J Physiol 450: 273-301.
April 18	Santana	Calcium-induced calcium release	Fabiato A. 1985. Time and calcium dependence of activation and inactivation of calcium- induced release of calcium from the sarcoplasmic reticulum of a skinned canine cardiac Purkinje cell. J Gen Physiol 85: 247-289
April 25	Santana	Local calcium signaling	Cheng et al. 1993. Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle. Science 262:740-744
May 6	--	Paper is due.		see instructions--

Course Evaluation

Last update April 8, 2011 5:18 PM