PBIO 550/NeuB 550 Biophysics of Calcium Signaling

Spring 2015

Meets: Mondays from 1:00 - 2:50 PM in G-417 of HSB

Instructors: Bertil Hille (hille@uw.edu; 3-8639, H-310G HSB) and
Luis Fernando Santana (santana@uw.edu; 3-0986, H-321 HSB)


2015 Lecture Schedule

date instructor topic discussion paper
April 27 Santana Fundamentals of fluorescence and intracellular Ca2+indicators Grynkiewicz et al. 1985. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450.
May 4 Hille Cellular calcium buffers and transport mechanisms.  Kinetics and rates. Neher E and Augustine GJ. 1992. Calcium gradients and buffers in bovine chromaffin cells. J Physiol 450: 273-301.
May 11 Santana Local Ca2+ signaling Sonkusare et al. Elementary Ca2+ signals through endothelial TRPV4 channels regulate vascular function Science 2012; 336:597-601
May 18 Hille Expressible calcium indicators
Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K. Characterization and subcellular targeting of GCaMP-type genetically-encoded calcium indicators. PLoS One. 2008 Mar 19;3(3):e1796.
May 25
Memorial Day Holiday
June 1 Santana Optogenetic regulation Ca2+ signaling Jia et al. Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery. Circulation. Arrhythmia and electrophysiology. 2011;4:753-760.
June 15   Your term paper is due.