PBIO 550/NeuB 550 Biophysics of Calcium Signaling

Spring 2009, first half of quarter (five weeks)

Instructors: Bertil Hille (hille@u.washington.edu; 3-8639, H-310G HSB) and
Luis Fernando Santana (santana@u.washington.edu; 3-0986, H-321 HSB)

Lecture schedule:

Date	Instructor	Topic (w/ link to PPT)	handouts	Paper (with link)
March 30	Hille	Cellular calcium buffers and transport mechanisms. Kinetics and rates.		Neher E and Augustine GJ. Calcium gradients and buffers in bovine chromaffin cells. J Physiol 450: 273-301, 1992.
April 6	Santana	Fundamentals of fluorescence and intracellular Ca2+ indicators		Grynkiewicz et al. 1985. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450.
April 13	Santana	Calcium-induced calcium release		Fabiato A. Time and calcium dependence of activation and inactivation of calcium- induced release of calcium from the sarcoplasmic reticulum of a skinned canine cardiac Purkinje cell. J Gen Physiol 85: 247-289., 1985.
April 20	Santana	Local calcium signaling		Cheng et al. 1993. Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle. Science 262:740-744.
April 27	Hille	Expressible calcium indicators		Palmer et al. Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. PNAS 101: 17404-17409, 2004.
May 4	--	Paper is due.		--