PBIO 550/NeuB 550 Biophysics of Calcium Signaling

Spring 2013, first half of quarter (five weeks)

Meets: Mondays from 1:00 - 2:50 PM in G-417 of HSB

Instructors: Bertil Hille (hille@uw.edu; 3-8639, H-310G HSB) and
Luis Fernando Santana (santana@uw.edu; 3-0986, H-321 HSB)


PBIO 550 course evaluation link


Course Schedule/Information [pdf file]

Lecture schedule:




Discussion Paper

April 1


Fundamentals of fluorescence and intracellular Ca2+ indicators

Grynkiewicz et al. 1985. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440-3450.

April 8


Expressible calcium indicators

Miyawaki A, et al (1999) Dyna­mic and quantitative Ca2+ measurements using improved cameleons. PNAS. 96:2135-40.

April 15


Optogenetic regulation Ca2+ signaling

Jia et al. Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery. Circulation. Arrhythmia and electrophysiology. 2011;4:753-760.

April 22


Cellular calcium buffers and transport mechanisms.  Kinetics and rates.

Neher E and Augustine GJ. 1992. Calcium gradients and buffers in bovine chromaffin cells. J Physiol 450: 273-301.

April 29


Local Ca2+ signaling

Sonkusare et al. Elementary Ca2+ signals through endothelial TRPV4 channels regulate vascular function Science 2012; 336:597-601

May 13


Paper is due.

see instructions


Course Evaluation

Last update June 21, 2013 4:59 PM