Monday, June 23:  Introduction to the cell

Required reading:  Alberts chapter 10, p. 617-629: the lipid bilayer

Interesting for those into fluorescent proteins: Shaner NC, Patterson GH, Davidson MW. Advances in fluorescent protein technology.J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60. PMID: 18057027 Link

Tuesday, June 24: Nucleocytoplasmic transport

Required review:  Terry LJ, Shows EB, Wente SR. Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transport. Science. 2007 Nov 30;318(5855):1412-6. PMID: 18048681 Link

Discussion Paper:  Satterly N, Tsai PL, van Deursen J, Nussenzveig DR, Wang Y, Faria PA, Levay A, Levy DE, Fontoura BM. Influenza virus targets the mRNA export machinery and the nuclear pore complex. Proc Natl Acad Sci U S A. 2007, 104(6):1853-8. PMID: 17267598 Link

Optional paper for writing assignment—Atasheva S, Garmashova N, Frolov I, Frolova E. Venezuelan equine encephalitis virus capsid protein inhibits nuclear import in Mammalian but not in mosquito cells. J Virol. 2008 Apr;82(8):4028-41. PMID: 18256144 .  Link 

Wednesday 3, June 25: The secretory system: principles of trafficking

Background Reading: Alberts p. 723-736; 750-765

Required review:  Mancias JD, Goldberg J.  Exiting the endoplasmic reticulum. Traffic. 2005 Apr;6(4):278-85. Review.PMID: 15752134 Link

Discussion paper: Gelsthorpe ME, Baumann N, Millard E, Gale SE, Langmade SJ, Schaffer JE, Ory DS.  Niemann-Pick type C1 I1061T mutant encodes a functional protein that is selected for endoplasmic reticulum-associated degradation due to protein misfolding. J Biol Chem. 2008 Mar 28;283(13):8229-36.  PMID: 18216017. Link

Optional paper for writing assignment: Chemical chaperones reduce ER stress and restore glucose homeostasis in a mouse model of type 2 diabetes. Science. 2006 ;313(5790):1137-40. MID: 16931765 Link

Additional reading.  Strongly recommended review. If you can't read it this week, try for next! Van Anken et al. 2005.  Versatility of the Endoplasmic reticulum folding factory.  Cr. Rev. Biochem.  Mol Bio 40:191-228. Link

Looking to the future--Pfeffer SR.  Unsolved mysteries in membrane traffic. Annu Rev Biochem. 2007;76:629-45. PMID: 17263661. Link

Thursday, June 26: Metabolic organelles:mitochondria

Background material:  Alberts, Chapter 12, p. 713-720.

Required review papers. Bohnert M, Pfanner N, van der Laan M. A dynamic machinery for import of mitochondrial precursor proteins. FEBS Lett. 2007 Jun 19;581(15):2802-10. Epub 2007 Mar 12. PMID: 17376437. Link

Discussion paper: Bradley PJ, Lahti CJ, Plümper E, Johnson PJ. Targeting and translocation of proteins into the hydrogenosome of the protest Trichomonas: similarities with mitochondrial protein import. EMBO J. 1997 Jun 16;16(12):3484-93.  PMID: 9218791 Link

Optional paper for writing assignment. Tsaousis AD, Kunji ER, Goldberg AV, Lucocq JM, Hirt RP, Embley TM. A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi.  Nature. 2008 Apr 30. PMID: 18449191 Link

Optional review for your interest.  MacKenzie JA, Payne RM. Mitochondrial protein import and human health and disease.  Biochim Biophys Acta. 2007 May;1772(5):509-23.  PMID: 17300922 Link

Friday, June 27:  Other metabolic organelles and function

Background reading: Alberts Chapter 12, p. 721-723 (peroxisomes)

Required Reviews: Platta HW, Erdmann R.  The peroxisomal protein import machinery. FEBS Lett. 2007 Jun 19;581(15):2811-9. Epub 2007 Apr 9. Review. PMID: 17445803 Link

Parsons M, Karnataki A, Feagin JE, DeRocher A.  Protein trafficking to the apicoplast: deciphering the apicomplexan solution to secondary endosymbiosis. Eukaryot Cell. 2007 Jul;6(7):1081-8. Epub 2007 May 18. PMID: 17513565 Link

Recommended: Ralph SA, van Dooren GG, Waller RF, Crawford MJ, Fraunholz MJ, Foth BJ, Tonkin CJ, Roos DS, McFadden GI.  Tropical infectious diseases: metabolic maps and functions of the Plasmodium falciparum apicoplast. Nat Rev Microbiol. 2004 Mar;2(3):203-16. Review. PMID: 15083156 Link