Cost of some items

Below are the costs of some items, together with a link to the company selling them. Protocols for their use can be found on the Protocols page.

Item Cost (US $) Notes
Course Fee 450  
Bench Fee 450 / month For work to be carried out in MMBL after the course
Quiagen extraction kits 2 / sample Needed for DNA extraction from tissues
Taq 175 / 500u need 1 u per PCR reaction, can be shared
unlabelled primers 25 / pair order from Operon, ask instructor for username and password
labelled primers 60 / pair only one labelled. FAM and HEX can be ordered from Operon, NED from Applied Biosystems (ABI) - have a look at their demo
PCR purification kits 2 / sample Needed to clean up your PCR product before sequencing
Sequencing kits 0.5 /reaction remember you need two reactions (forward & reverse) / sequence
MegaBace run 140 / run consists of 96 wells; can be shared
Size standards 20 / run only needed for genotyping (microsatellites)
Cloning Kits 398 / 20 reactions can be shared