Below are the costs of some items, together with a link to the company selling them. Protocols for their use can be found on the Protocols page.
Item | Cost (US $) | Notes |
Course Fee | 450 | |
Bench Fee | 450 / month | For work to be carried out in MMBL after the course |
Quiagen extraction kits | 2 / sample | Needed for DNA extraction from tissues |
Taq | 175 / 500u | need 1 u per PCR reaction, can be shared |
unlabelled primers | 25 / pair | order from Operon, ask instructor for username and password |
labelled primers | 60 / pair | only one labelled. FAM and HEX can be ordered from Operon, NED from Applied Biosystems (ABI) - have a look at their demo |
PCR purification kits | 2 / sample | Needed to clean up your PCR product before sequencing |
Sequencing kits | 0.5 /reaction | remember you need two reactions (forward & reverse) / sequence |
MegaBace run | 140 / run | consists of 96 wells; can be shared |
Size standards | 20 / run | only needed for genotyping (microsatellites) |
Cloning Kits | 398 / 20 reactions | can be shared |