Oceanography 443, Winter 2010
Design of Oceanographic Field Experiments


Shared equipment and consumables

A special note from Kathy Newell:

Here are some suggestions for starting your search for supplies. You may have to order from specialty companies for some items:


Biochemistry Stores - http://128.95.12.98:88/stores/dataSearch.asp


VWR Catalog - http://www.vwr.com


Fisher Catalog - http://fishersci.com


Please submit orders to me (kknewell@u.washington.edu) with the following information:


Name & email
Source
Item
Catalog or reference number
Quantity
Cost


I do have a supply of chemicals, filters and glassware in storage, so check with me before trying to find a vendor.


Here is the equipment you can reserve from me:


Microscopes, dissecting scopes, camera, fluorometer, filtration rack, sonicator and centrifuge.


Please allow adequate time for ordering. Some items may be special order or some may be backordered.
DON'T WAIT UNTIL THE DAY BEFORE YOUR CRUISE TO PLACE YOUR ORDER AND EXPECT EVERYTHING TO SHOW UP ON TIME.




UW Biochemistry Stores
UW Central Stores
VWR Catalog
Fisher Catalog

Measurements and Equipment

The Thompson is permanently equipped with an ADCP (75 kHz), a dual-frequency (12 and 3.5 kHz) echo sounder/subbottom profiler, a Simrad EM300 (30 kHz) swath mapping system, a CTD system, and GPS positioning systems. The CTD system includes a transmissometer, fluorometer, O2 sensor, PAR sensor, altimeter, and up to twenty-four 10 L Niskin bottles. Other sampling equipment must be requested and rented from Pooled Equipment. Additional laboratory equipment (bench-top fluorometer, filter rack and pump, microscopes, glassware, etc.) is available through Classroom Services (Kathy Newell, 543-6119, 21 OTB). Our Marine Chemistry Lab can provide a wide range of analytical services.

Below is a summary of possible measurements and available equipment relevant to the March Chile cruise. If your research project requires additional materials, please contact the course professors. It is possible that the materials will be available, either via our collaborators, or because they were overlooked in the following list! Items listed below but not found among Pooled Equipment can be assumed to have been donated and, for the purposes of your proposal budgets, are therefore free.

Additional items may be added so check back for updates.


Measurements:

Phytoplankton biomass (chl a), abundance, size distribution, and species composition

Zooplankton biomass, abundance, size distribution, and species composition

Bacterial and viral abundance

Phytoplankton growth rates (via dilution experiments)

Phytoplankton production (via oxygen production experiments)

Diatom silicification (via biogenic silica and fluorescent staining)

Grazing rates on phytoplankton (via dilution experiments [microzooplankton] or other bottle experiments [mesozooplankton, like copepods]

Virus-induced mortality of phytoplankton (via dilution experiments)

Grazing rates OF (in most cases, not ON) zooplankton

Growth rates of bacteria (via dilution experiments)

Grazing rates on bacteria (via dilution experiments)

Virus-induced mortality of bacteria (via dilution experiments)

Virus production

Macronutrient analyses (nitrate, phosphate, silicate, ammonia)

Salinity analyses

Oxygen analyses

Water column profiling (salinity, temperature, fluorescence, photosynthetically active radiation and transmissivity)

Seafloor mapping (with the Simrad EM300)

Current profiling (with the shipboard ADCP)



Shipboard equipment:

3 incubators, each capable of holding ~18 2-L bottles

Various filtration rigs, for preparation of samples of chl a (including size-fractionated), bacteria and viruses

Dissecting, compound and epifluorescent microscopes for analysis of zooplankton, phytoplankton, bacteria and viruses

Balances, for measuring weight

Fluorometer, for measuring chl a

6 cubitainers, 24 500-ml bottles, and 2 L clear bottles (for incubations)

36-32 oz. jars, 24-4 oz. jars, 48-8 oz. jars

Oxygen bottles and Winkler system

Oxygen microelectrode

nutrient and salinity bottles

Assorted nets: Two phytoplankton nets with 64 or 25-micron meshes; one1-m-diameter net 500-micron mesh; two half meter-diameter closing vertical net with 216-micron meshes

On deck incubator

Soutar corer, Multicorer, Gravity Corer, Kasten Corer

Secchi disk

CTD rosette with Niskin bottles for water-column sampling

ADCP for current profiling

Simrad EM300 for bottom mapping

Magnetometer