Syllabus

Very tentative - may change

Wk Date Lecture (FTR 103) Lab (FTR 129) Assignments
1 Tue
Jan 6		 Cancelled Cancelled Read Homepage
Th
Jan 8		 Intro, course stuff,
DNA storage and extraction

Pipetting,
Buffers and concentrations
Tour of MMBL
Set up Prot K digest
2 Tue
Jan 13

PCR troubleshooting

DNA extraction (Quiagen, Salt)
PCR (mtDNA, microsatellites)
Th
Jan 15		 eletrophoresis,
DNA quantification

Agarose gel (HMW DNA), SybrGold staining, Set up RFLP,

Agarose gel pictures with legends (powerpoint); gel1, gel2, gel3, gel4, gel5

 Proposal due
3 Tue
Jan 20		 Cloning

Cloning (ligation, transformation, growing)

  
Wed pick colonies
Th
Jan 22		   Miniprep, GeneQuant, Sequencing reaction Review due
4 Tue
Jan 27		 Sequencing

Clean up reaction,
sequencing, pour PAGE gel

Sequencing results (5 MB)

  
Th
Jan 29		 Microsatellites

PAGE (mtDNA RFLP, microsatellites), FMBIO

PAGE Gels

  
5 Tue
Feb 3

Sequencing feedback,
Proposal Feedback

 Independent project work starts  
Th
Feb5		  
6 Tue
Feb 10		 Group discussion
Th
Feb 12		  
7 Tue
Feb 17		 Mid Term meeting
Th
Feb 19		  
8 Tue
Feb 24		 Group discussion  
Th
Feb 26		  
9 Tue
Mar 2		 Group discussion
Th
Mar 4		  
10 Tue
Mar 9		 Group discussion
Th
Mar 11		  
11

Tue
March 16

 Final presentation
Th
March 18		   Final report due