Autumn
2009

FISH/BIOL 340 - Genetics and Molecular Ecology

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Home

Instructors
Instructors

Course Description
Course Description

Syllabus
Syllabus

Grading
Grading

Labs
Labs

Project
Lab Project

Required Reading
Reading

Glossary
Glossary of Terms

Links
Links

 

Syllabus

The course will consist of two 80 min lectures (T,Th) and one 3 hour lab class (2 sections, Th F pm) per week. Some lectures will be given by guest speakers, who will present case studies of genetic applications in their field.

The class will include both practical and computer labs (see also the Labs page. In the computer laboratories we will use simulation programs to learn about genetic processes and analysis programs to analyze sample data sets. In the practical laboratories we will conduct a study identifying the species of origin of rockfish fillets sold in Seattle markets (see lab project).

Below is a tentative timetable, including due dates for assigments (Assign.). Please note that the timing of specific topics may change in the course of the quarter - in fact it usually does, as I want to make sure that everybody understands the material and so am more than happy to explain issues again. In addition, time conflicts of guest speakers have to be accommodated. Links in each lecture point to the PowerPoint file of slides I used in class. Final versions of each lecture will be posted here on the day of the previous lecture - if you want a copy during lecture, please print out yourself.

W

D

 

L

Lecture or Lab

Assignments/Due dates

1

Th

10/1

1

Introduction, Class structure, Project background

Read web page

 

Lab

 

 

DNA extraction, pipetting

Read project report instructions, postlab questions

2

Tu

10/6

2

DNA, replication, PCR, Microsatellites

Hallerman et al. 2003

 

Th

10/8

3

Mendelian inheritance

 

 

Lab

 

 

Parentage

Feedback on Results section

3

Tu

10/13

4

Mendelian inheritance II

population genetics chapter

 

Th

10/15

5

Hardy Weinberg equilibrium

 

 

Lab

 

 

PCR & RFLP

4

Tu

10/20

6

Microevolutionary forces I: drift

Early Quiz

 

Th

10/22

7

Effective population size

 

Lab

 

 

Hardy Weinberg equilibrium

5

Tu

10/27

8

Microevolutionary forces II: mutation

 

Th

10/29

9

Microevolutionary forces III: selection

 

Lab

 

 

RFLP electrophoresis

6

Tu

11/3

 

Mid Term Exam

Mid Term Exam key

 

Th

11/5

10

Microevolutionary forces IV migration

 

 

Lab

 

 

Population genetic analysis

7

Tu

11/10

11

Migration

Molecular evolution chapter

 

Th

11/12

12

Of trees and bushes

 

Lab

 

 

GenBank & tree construction

8

Tu

11/17

13

phylogeography

 

Th

11/19

14

Allozymes

 

 

Lab

 

 

Allozymes

 

9

Tu

11/24

15

Recombinant DNA

 

 

Th

11/26

 

Thanksgiving

 

 

Lab

 

 

Thanksgiving

10 Tu 12/1 16 Genomics & Metagenomics  
  Th 12/3 17 Adaptive genetic variation  

 

Lab

 

 

Cloning

Final Project Report Due in Lab

11

Tu

12/8

18

The future  
  Th 12/10 19 TBD  
  Lab     Cloning and Review  

 

Mo

12/14

 

Final Exam (10:30-12:20, FSH 107)